phytohemagglutinin function in cell culture

  • Phytohemagglutinin: An Initiator of Mitosis in Cultures of Normal

    leukocytes which had been separated from whole blood by the usual PHA method. These cells were planted in cultures in which the liquid phase was replaced 

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  • Phytomitogens and lymphocyte blastogenesis | SpringerLink

    A method for the rapid separation of leukocytes and nucleated erythrocytes from Phytohaemagglutinin and human lymphocytes in short-term cell culture.

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  • Interleukin (IL)-13 Production by Phytohemagglutinin (PHA - JACI

    by Phytohemagglutinin (PHA)-stimulated Peripheral Blood Mononuclear Cells from PHA-stimulated PBMC and total IgE, asthma severity, and lung function in IL-13 concentration in culture supernatants was measured by Luminex bead 

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  • Human T cell activation with phytohemagglutinin. The function of IL

    Dec 1, 1988 Addition of a cell-free monocyte culture supernatant (not containing IL-2) to the PHA-stimulated cultures resulted in T cell proliferation.

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  • PDF LinkPDF

    toincompatible lymphocytes in vitro, the mixed leukocyte culture (MLC) 1 (7-11). of PHA-induced lymphocyte proliferation by monocytes (11), whereas Oppenheim pendent (B) cells on the basis of ontogeny, function, and cell surface 

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  • LPMGF - Clinical: Lymphocyte Proliferation to Mitogens, Blood

    LPMGF : The method of determining impaired T-cell function by culturing human lectins (mitogens) such as phytohemagglutinin (PHA) and pokeweed mitogen 

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  • Comparative Response of Normal Human Thyinus and Lymph Node

    Node. Cells to Phytohemagglutinin in Culture. By ALAN. WINKELSTEIN. AND function as immunologically competent lymphocytes in the periph- cry.14.

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  • PHA (Phytohaemagglutinin P) for T cells activation | InvivoGen

    The significance appliion of indigenous Phytohemagglutinin (PHA) mitogen on metaphase and cell culture procedure. Iran J Pharm Res. 10(4):895-903.

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  • Phytohemagglutinin and Concanavalin A - Infection and Immunity

    the mitogens concanavalin A and phytohemagglutinin. prevent the production or function of cell-me- Spleen cell cultures were also prepared from unin-.

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  • Inhibition of phytohemagglutinin-induced lymphocyte mitogenesis

    the functional importance of the OH and carboxylic functions was tested using 1640 tissue culture media, HEPES, and penicillin-strep- tomycin antibiotics were  

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  • Targeting and Release of Phytohemagglutinin from the Roots of

    We suggest that this cell-wall. PHA is the source of the PHA in the culture medium. Phytohemagglutinin (PHA), an abundant vacuolar seed protein of.

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  • Phytohaemagglutinin - ScienceDirect.com

    A common method of retrieving PHA is to disrupt the cells, collect the cellular PHBs have been successfully used as films in cell culture studies [51] and as 

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  • The rapid induction by phytohemagglutinin of increased a

    The change in membrane function developed even when de novo When fetuin was added to lymphocyte cultures to remove bound PHA, the PHA-induced increase in the tion, and that the continued presence of PHA on the cell membrane 

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  • Human lymphocyte culture/ Phytohemagglutinin / Lectin - J-Stage

    Human lymphocyte culture/ Phytohemagglutinin / Lectin / Mitogens /Mitotic index were quite limited, and the purpose of the present study is to establish the most After the addition of mitogens, the cells were cultured at 37°C for 50 hours 

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  • Functional impairment of human T-lymphocytes following PHA

    Oct 3, 2002 However, little functional analysis of PHA stimulation is available. Our results show that expansion of T cells with PHA impairs their ability to Positive control cultures were performed using fresh PBMCs from the same donors.

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  • Human T cell activation with phytohemagglutinin. The function of IL

    Dec 1, 1988 Addition of a cell-free monocyte culture supernatant (not containing IL-2) to the PHA-stimulated cultures resulted in T cell proliferation.

    Chat Online
  • Phytohaemagglutinin - ScienceDirect.com

    A common method of retrieving PHA is to disrupt the cells, collect the cellular PHBs have been successfully used as films in cell culture studies [51] and as 

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  • Simplifying Lymphocyte Culture and the - Clinical Chemistry

    Cultures (with PHA-P) from 10 Normal Subjects. E3H]TdR method previously described. (3). caused difficulty in washing the cells and in removing hemo-.

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  • (PHA-M) Instructions for Use - Biological Industries

    Product Description. Phytohaemagglutinin is a lectin extracted from red kidney a crude extract used for the stimulation of cell proliferation in lymphocyte culture. PHA-M also has a The most common method to obtain this staining is to treat 

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  • Phytohemagglutinin-L (PHA-L) from Phaseolus vulgaris | Sigma

    Phytohemagglutinin-L (PHA-L) is a lectin extract that binds to T-cell is well suited for high-efficiency induction and the functional analysis of T-lymphocytes.

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